Pharmacological evaluation of anti-inflammatory potential and antispasmodic effect of aqueous extract from the brown seaweed,Cystoseiracompressa

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Published Apr 12, 2026
https://doi.org/10.62438/tunismed.v104i03.5980
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Vol. 104 No. 03 (2026): Tunis Med Mar 2026

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Mohamed Amine El Kaibi
Laboratory of Drug Development (LR12ES09), Faculty of Pharmacy, University of Monastir, 5000 Monastir, Tunisia
Mariem Lameri
Laboratory of Drug Development (LR12ES09), Faculty of Pharmacy, University of Monastir, 5000 Monastir, Tunisia
https://orcid.org/0009-0000-6856-0483
Rihab Ayadi
Laboratory of Drug Development (LR12ES09), Faculty of Pharmacy, University of Monastir, 5000 Monastir, Tunisia
https://orcid.org/0009-0000-3395-5338
Safa Amri
Laboratory of Drug Development (LR12ES09), Faculty of Pharmacy, University of Monastir, 5000 Monastir, Tunisia
Abderrahman Bouraoui
Laboratory of Drug Development (LR12ES09), Faculty of Pharmacy, University of Monastir, 5000 Monastir, Tunisia
https://orcid.org/0000-0002-7881-6574

Abstract

Introduction: Gastrointestinal (GI) pain is commonly treated with synthetic spasmolytic drugs, including anticholinergics, smooth muscle relaxants,
calcium channel blockers, and opioid receptor modulators. However, these treatments often lead to undesirable side effects.
Aim: To evaluate the antispasmodic and anti-inflammatory potential of the aqueous extract from the brown seaweed Cystoseira compressa (Comp-
AQ).
Methods: The antispasmodic activity of Comp-AQ was assessed in vitro using rat duode-num contractions induced by acetylcholine (A/Ch)
and barium chloride (BaCl₂). The anti-inflammatory activity was evaluated in vivo using the carrageenan-induced paw edema model in rats. A phytochemical screening was performed to identify bioactive compounds, and antioxidant activity was assessed via the DPPH radical scavenging assay.
Results: Comp-AQ significantly and dose-dependently inhibited A/Ch- and BaCl₂-induced contractions (82.98 ± 1.5% and 84.06 ± 2.16%, respectively), with a relaxation-response profile similar to alverine, suggesting involvement of voltage-dependent calcium channels. In vivo, Comp-AQ reduced carrageenan-induced paw edema, reaching a maximal inhibition of 71.46% at 100 mg/kg after 3 hours. Phytochemical analysis revealed a high content of marine polyphenols and polysaccharides. The extract exhibited strong antioxidant activity with an IC₅₀ of 30 ± 2.66 µg/mL.
Conclusion: The aqueous extract of Cystoseira compressa exhibits antispasmodic, anti-inflammatory, and antioxidant properties. Further structural
characterization is needed to definitively identify the re-sponsible bioactive compounds and to confirm the hypothesis of synergistic effects.

Keywords:

Seaweed , Cystoseira compressa, Antispasmodic activity , Anti-inflammatory activity , Marine polyphenols

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